scRNA-seq Profiling of Human Testes Reveals the Presence of the ACE2 Receptor, A Target for SARS-CoV-2 Infection in Spermatogonia, Leydig and Sertoli Cells.
Identifieur interne : 000018 ( 2020/Analysis ); précédent : 000017; suivant : 000019scRNA-seq Profiling of Human Testes Reveals the Presence of the ACE2 Receptor, A Target for SARS-CoV-2 Infection in Spermatogonia, Leydig and Sertoli Cells.
Auteurs : Zhengpin Wang [États-Unis] ; Xiaojiang Xu [États-Unis]Source :
- Cells [ 2073-4409 ] ; 2020.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Humains, Infections à coronavirus (transmission), Infections à coronavirus (virologie), Mâle, Pandémies, Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (métabolisme), Petit ARN cytoplasmique (), Petit ARN cytoplasmique (génétique), Pneumopathie virale (transmission), Pneumopathie virale (virologie), Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Régulation de l'expression des gènes, Réplication virale (génétique), Testicule (cytologie), Testicule (métabolisme), Testicule (virologie).
- MESH :
- cytologie : Testicule.
- génétique : Peptidyl-Dipeptidase A, Petit ARN cytoplasmique, Récepteurs viraux, Réplication virale.
- métabolisme : Peptidyl-Dipeptidase A, Récepteurs viraux, Testicule.
- virologie : Infections à coronavirus, Pneumopathie virale, Testicule.
- Analyse de profil d'expression de gènes, Humains, Infections à coronavirus, Mâle, Pandémies, Petit ARN cytoplasmique, Pneumopathie virale, Régulation de l'expression des gènes.
English descriptors
- KwdEn :
- Coronavirus Infections (transmission), Coronavirus Infections (virology), Gene Expression Profiling, Gene Expression Regulation, Humans, Male, Pandemics, Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), Pneumonia, Viral (transmission), Pneumonia, Viral (virology), RNA, Small Cytoplasmic (chemistry), RNA, Small Cytoplasmic (genetics), Receptors, Virus (genetics), Receptors, Virus (metabolism), Testis (cytology), Testis (metabolism), Testis (virology), Virus Replication (genetics).
- MESH :
- chemical , chemistry : RNA, Small Cytoplasmic.
- chemical , genetics : Peptidyl-Dipeptidase A, RNA, Small Cytoplasmic, Receptors, Virus.
- chemical , metabolism : Peptidyl-Dipeptidase A, Receptors, Virus.
- cytology : Testis.
- genetics : Virus Replication.
- metabolism : Testis.
- transmission : Coronavirus Infections, Pneumonia, Viral.
- virology : Coronavirus Infections, Pneumonia, Viral, Testis.
- Gene Expression Profiling, Gene Expression Regulation, Humans, Male, Pandemics.
Abstract
In December 2019, a novel coronavirus (SARS-CoV-2) was identified in COVID-19 patients in Wuhan, Hubei Province, China. SARS-CoV-2 shares both high sequence similarity and the use of the same cell entry receptor, angiotensin-converting enzyme 2 (ACE2), with severe acute respiratory syndrome coronavirus (SARS-CoV). Several studies have provided bioinformatic evidence of potential routes of SARS-CoV-2 infection in respiratory, cardiovascular, digestive and urinary systems. However, whether the reproductive system is a potential target of SARS-CoV-2 infection has not yet been determined. Here, we investigate the expression pattern of ACE2 in adult human testes at the level of single-cell transcriptomes. The results indicate that ACE2 is predominantly enriched in spermatogonia and Leydig and Sertoli cells. Gene Set Enrichment Analysis (GSEA) indicates that Gene Ontology (GO) categories associated with viral reproduction and transmission are highly enriched in ACE2-positive spermatogonia, while male gamete generation related terms are downregulated. Cell-cell junction and immunity-related GO terms are increased in ACE2-positive Leydig and Sertoli cells, but mitochondria and reproduction-related GO terms are decreased. These findings provide evidence that the human testis is a potential target of SARS-CoV-2 infection, which may have significant impact on our understanding of the pathophysiology of this rapidly spreading disease.
DOI: 10.3390/cells9040920
PubMed: 32283711
Affiliations:
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pubmed:32283711Le document en format XML
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a novel coronavirus (SARS-CoV-2) was identified in COVID-19 patients in Wuhan, Hubei Province, China. SARS-CoV-2 shares both high sequence similarity and the use of the same cell entry receptor, angiotensin-converting enzyme 2 (ACE2), with severe acute respiratory syndrome coronavirus (SARS-CoV). Several studies have provided bioinformatic evidence of potential routes of SARS-CoV-2 infection in respiratory, cardiovascular, digestive and urinary systems. However, whether the reproductive system is a potential target of SARS-CoV-2 infection has not yet been determined. Here, we investigate the expression pattern of ACE2 in adult human testes at the level of single-cell transcriptomes. The results indicate that ACE2 is predominantly enriched in spermatogonia and Leydig and Sertoli cells. Gene Set Enrichment Analysis (GSEA) indicates that Gene Ontology (GO) categories associated with viral reproduction and transmission are highly enriched in ACE2-positive spermatogonia, while male gamete generation related terms are downregulated. Cell-cell junction and immunity-related GO terms are increased in ACE2-positive Leydig and Sertoli cells, but mitochondria and reproduction-related GO terms are decreased. These findings provide evidence that the human testis is a potential target of SARS-CoV-2 infection, which may have significant impact on our understanding of the pathophysiology of this rapidly spreading disease.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Wang, Zhengpin" sort="Wang, Zhengpin" uniqKey="Wang Z" first="Zhengpin" last="Wang">Zhengpin Wang</name></region><name sortKey="Xu, Xiaojiang" sort="Xu, Xiaojiang" uniqKey="Xu X" first="Xiaojiang" last="Xu">Xiaojiang Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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